Click on the GCGR Cell line ID to view the datasheet, which also has cell images, transplantation data, flow cytometry analysis and STR profile
| GCGR Cell line ID | Description | Method used to generate reporter line |
Classification of tumour tissue |
GBM Transcriptional subclass of GSC line | Molecular data available for parental line |
Tumour formation following transplant of GFP-Luc cell line |
|
|---|---|---|---|---|---|---|---|
| Classical/Proneural/Mesenchymal (Wang et al., Cancer Cell 32, 42-56 2017) |
MESImm/Non-MESImm (Gangoso et al., Cell 184, 2454-2470 2021) |
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| GCGR-E3_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~10 months) |
| GCGR-E13_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Classical | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~5 months) |
| GCGR-E15_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Not assigned | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~4 months) |
| GCGR-E17_PB-GFP_Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Classical | MESImm/Non-MESImm equal | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~7 months) |
| GCGR-E20_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Proneural | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~4 months) |
| GCGR-E21_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | 40% mortality at 1 year |
| GCGR-E22_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~10 months) |
| GCGR-E24_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Proneural | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~8 months) |
| GCGR-E25_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~8 months) |
| GCGR-E28_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~6 months) |
| GCGR-E30_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~11 months) |
| GCGR-E34_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Proneural | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | No lethal brain tumours at 1 year |
| GCGR-E35_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~9 months) |
| GCGR-E39_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | No lethal brain tumours at 1 year |
| GCGR-E43_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~3 months) |
| GCGR-E53_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Proneural | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~7 months) |
| GCGR-E54_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Proneural | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | 20% mortality at 5 months |
| GCGR-E55_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~4 months) |
| GCGR-E56_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~5 months) |
| GCGR-E63_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~4 months) |
| GCGR-E64_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | No lethal brain tumours at 1 year |
| GCGR-E65_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | No lethal brain tumours at 1 year |
| GCGR-L5_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~4 months) |
| GCGR-L7_PB-GFP_Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays" | Yes (~6 months) |
| GCGR-L11_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Not assigned | MESImm/Non-MESImm equal | "RNA-seq, EPIC methylation arrays" | Yes (~9 months) |
| GCGR-L12_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | No lethal brain tumours at 1 year |
| GCGR-L15_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~10 months) |
| GCGR-L19_PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Not attempted |
| GCGR-L21_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~6 months) |
| GCGR-L24_AAVS1-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | Knockin at AAVS1 safe harbour locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Not assigned | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~6 months) |
| GCGR-NS9FB_B-PB-GFP-Luc | Constitutive expression of Luciferase-T2A-eGFP under control of CAG promoter | PiggyBac transposase random insertion | "N/A, hNSC line" | N/A | N/A | "RNA-seq, EPIC methylation arrays, WGS" | No lethal tumours at 1 year |
| GCGR-E13_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~5 months) |
| GCGR-E15_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Not assigned | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~4 months) |
| GCGR-E17_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | MESImm/Non-MESImm equal | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~7 months) |
| GCGR-E24_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Proneural | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~8 months) |
| GCGR-E25_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~8 months) |
| GCGR-E26_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~9 months) |
| GCGR-E27_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Not assigned | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | No lethal brain tumours at 1 year (cells on top of brain ~8 months) |
| GCGR-E30_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~11 months) |
| GCGR-E31_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Proneural | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~10 months) |
| GCGR-E35_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~9 months) |
| GCGR-E37_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~4 months) |
| GCGR-E43_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~3 months) |
| GCGR-E54_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Mesenchymal | MESImm | "RNA-seq, EPIC methylation arrays, WGS" | 20% mortality at 5 months |
| GCGR-E56_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Classical | Non-MESImm | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~5 months) |
| GCGR-E62_SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "Glioblastoma, IDH wildtype" | Not assigned | MESImm/Non-MESImm equal | "RNA-seq, EPIC methylation arrays, WGS" | Yes (~6 months) |
| NS9FB_B-SOX2-mCherry | Expression of SOX2-mCherry fusion protein under control of endogenous SOX2 | Knockin at SOX2 locus using CRISPR/Cas9 | "N/A, hNSC line" | N/A | N/A | "RNA-seq, EPIC methylation arrays, WGS" | No lethal tumours at 1 year |